Can anyone tell me why you would choose an antibody conjugated to any horseradish peroxidase or alkaline peroxidase over the other for use in different types of ELISA?
HRP tend to be faster and more sensitive, but it's substrates tend to be more toxic and the reaction will hold back itself. AP is a bit slower, but sensitivity can be increased by longer incubation times since the enzyme won't stop itself. it also depends on what reagents and detection methods you have handy. :)
medication and health guarantee correctness , is for informational purposes solely
Related Questions and Answers
No comments:
Post a Comment